Neutron Study of Cytochrome P450 Reductase: The Complex With Cytochrome c in Solution
Electron transfer from cytochrome P450 reductase to cytochrome P450 can only be observed when both proteins are bound to a membrane, and cytochrome c is widely used as a surrogate electron acceptor for studies in solution. Previous studies have looked at the conformation of CPR in solution alone with respect to redox state, this experiment is aimed to see the conformation adopted by the enzyme in the presence of an electron transfer partner. The work proposed is based off of data obtained in experiment TEST-2457 in May 2015. The data obtained from the experiment was very positive and permitted useful conclusions to be drawn, further extending the understanding of the fundamentals of the system. A publication is currently in the writing stage based on the obtained data. As a result of the data the study can be advanced in to looking at CPR in in a complex with an electron transfer partner, cytochrome c. This will require the use of deuteration facilities (proposal accepted) for deuteration of CPR.
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FREEMAN Samuel; MARTEL Anne; PORCAR Lionel and RAVEN Emma. (2016). Neutron Study of Cytochrome P450 Reductase: The Complex With Cytochrome c in Solution. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-878
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