Investigating conformational changes of BmrA reconstituted into invisible-in-SANS artificial nanoscale bilayer disc carriers
The ABC transporter BmrA is involved in the efflux of drugs and antibiotics in Bacillus subtilis (e.g. ethidium, doxorubicin, cervimycin C). Large conformational changes must occur to permit substrate translocation. Our aim is to characterize different conformations of BmrA. We obtained very promising SANS data on detergent-solubilized WT and mutant BmrA blocked in different conformational states. We now aim at studying BmrA reconstituted into artificial nanoscale bilayer discs that mimic the native bilayer environment. In the deuterated form, they should be invisible to neutrons in 100% D2O.
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EBEL Christine; BREYTON Cecile; JAULT Jean Michel; JAVED Waqas; MARTEL Anne; ORELLE Cedric and VALLET Sylvain. (2020). Investigating conformational changes of BmrA reconstituted into invisible-in-SANS artificial nanoscale bilayer disc carriers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-957
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