Solid-to-fluid like DNA transition in phage capsid controls viral infectivity. Revealing structure of intra-capsid DNA in phage with SANS
Bacteriophage λ (phage λ) is a virus which infects Escherichia coli (E. coli) by injecting viral double-stranded (ds) DNA into the bacterial cytoplasm without capsid disassembly. The ejection dynamics was recently found define the course of the infection. Rapid synchronized DNA injections from tens of pages into a single host cell prompts a latent infection in which the viral DNA is integrated into the host without affecting its vital functions. At the same time, slow desynchronized injections lead to lytic infection, where new viruses are reproduced within the cell leading to cell lysis and release of new infectious virions. The encapsidated DNA undergoes a structural transition which seems evolutionary adapted to increase the mobility of DNA and facilitate ejection but the pre- and post-transition packed structure is unknown. We want to use neutrons to study the specifics of the genome structure inside the Phage λ and how this structure and structural transitions affect the dynamics of virus infection and replication or latency.
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Alex Evilevitch; Adrian Gonzalez; SCHWEINS Ralf; Stephanie Tassler and TSIMTSIRAKIS Efthymios. (2019). Solid-to-fluid like DNA transition in phage capsid controls viral infectivity. Revealing structure of intra-capsid DNA in phage with SANS. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-960
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