Structural studies of integral membrane proteins in Salipro stealth carriers
Reconstitution of integral membrane proteins (IMPs) in nanodiscs or Salipro (saposin-lipid-protein) complexes allows low-resolution structural investigation of membrane proteins in a native-like environment. In order to fully contrast-match-out the nanodisc carriers, we have recently incorporated our IMP targets in stealth carriers consisting of deuterated MSP1 belt protein and deuterated lipids. This allowed the structural characterization of our IMPs of interest in a lipid environment without the often-dominating scattering contribution of the nanodisc (Josts et al, 2018, Structure). We now further plan to adapt the stealth carrier system to the Salipro system by selectively deuterating saposin A and thus being able to contrast match out the scaffold proteins/lipids also in the Salipro system.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
TIDOW Henning; FORSYTH Victor Trevor; JOSTS Inokentijs; KEHLENBECK Dominique Maurice; NITSCHE Julius and PREVOST Sylvain. (2019). Structural studies of integral membrane proteins in Salipro stealth carriers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-969
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.