Structural determination of mTSPO/detergent complexes
Our project is to determine the solution structures of TSPO (translocator protein outer membrane), an ubiquitous and functionally important membrane protein involved in neurodegeneration and cancer in human. No XR crystallography structure is available for the mammal form (mouse mTSPO) of this protein. The objective is to characterize the interactions between mTSPO and detergents, by coupling molecular dynamics simulations and scattering experiments, in order to better understand the influence of the surfactant on the crystallization of the membrane complexes. We would like to measure mTSPO with SDS (its solubilisation detergent) or DPC (used to solve its NMR structure). We will use different contrast matching conditions to probe selectively the whole complex, the detergent belt or the protein alone. We will need to combine SANS with the size-exclusion chromatography (SEC-SANS), a unique setup available at the ILL. With membrane proteins, the chromatographic setup is absolutely necessary to separate the protein/detergent complexes from the detergent free micelles.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
COMBET Sophie; Bonneté; finet; LACAPERE Jean Jacques; MARTEL Anne and POZZA Alexandre. (2020). Structural determination of mTSPO/detergent complexes. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-993
This data is not yet public
This data is not yet public
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