Small-Angle Neutron Scattering studies of bacterial colonization factors
Cholera is an ancient and deadly diarrheal disease that is caused by the pathogen Vibrio cholerae. The bacterium can easily survive in the ocean, where it binds to plankton and crustaceans. Upon ingestion of contaminated water or food, the bacteria colonize the human small intestine, where they secrete their major virulence factor, the cholera toxin. Currently, many of these processes are poorly understood. Insight into the underlying molecular mechanisms may help us to develop new medications, which are urgently needed given the increase in antibiotic resistance. This proposal targets the bacterial adhesin N-acetyl glucosamine binding protein A (GbpA) and its interaction with chitin, which we plan to study with Small-Angle Neutron and X-ray Scattering. This is a continuation of proposal 8-03-988, taking place in September (after proposal submission). After completion of the experiments with GbpA and chitin, we plan to extend the work to GbpA homologues from other bacteria and viruses.
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KRENGEL Ute; Matthew P. Blakeley; CARDENAS; Yubexi Correa; Kira D. Leitl; PREVOST Sylvain; SORENSEN Henrik Vinther and TISLEVOLL Abelone. (2020). Small-Angle Neutron Scattering studies of bacterial colonization factors. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-994
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