Deep sea microbes under high pressure
In the past, neutron experiments performed on bacteria and purified proteins proved to be successful in quantifying the extent to which macromolecular dynamics is affected by adaptation to extreme temperatures (Tehei et al., 2004 and 2005). Dynamics fluctuations and force constants were found by recording elastic incoherent neutron scattering on IN13. The results suggested that adaptation occurs by selecting an appropriate resilience in order to maintain optimal macromolecular flexibility required for enzyme activity while modifying protein rigidity to prevent unfolding in extreme temperature conditions. High pressure conditions prevail in a vast part of the biosphere. The existence of a pressure adaptation at the molecular dynamics level is still not established. To address this question, we will explore the effects of hydrostatic pressure on the macromolecular dynamics of different micro organic species adapted and non-adapted to high-pressure conditions (shallow and deep sea area). The present proposal is part of a funded four years scientific project (ANR Living Deep 2010-2014). The experiments proposed in this proposal are mandatory, together with the data collection already
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FRANZETTI Bruno; JEBBAR Mohamed; MARTINEZ Nicolas; MICHOUD Gregoire; Phil Oger and PETERS Judith. (2013). Deep sea microbes under high pressure. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-693
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