Discerning static from transient clusters using neutron spin echo and backscattering
The presence of dynamic or transient clusters in protein solutions and its understanding in terms of colloid physics is of great current interest due its relevance for pathological pathways as well as for drug design [K.P.Johnston et al., ACS Nano 6, 1357 (2012)]. Earlier studies combining SAXS and neutron spin-echo have addressed this issue using the model protein lysozyme [L.Porcar et al., J.Phys.Chem.Lett. 1, 126 (2010)]. Here we propose a systematic study of the cluster formation in aqueous solutions of the new model protein beta-lactoglobulin (BLG). We propose to measure the diffusion function for several values of q below and above q_c using IN15. We have previously obtained the short-time self-diffusion of BLG as a function of volume fraction phi in D2O indicating the presence of static clusters with a size that depends on phi. Moreover, we have obtained the diffusion functions for two samples on IN11, which, interestingly, disagree with the expectation of static clusters and do not converge to the short-time diffusion at high q. To resolve the arising questions, we clearly need IN15. Due to the prevailent colloid physics aspects, we explicitly submit to college 9.
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GRIMALDO Marco; FEUSTEL Michal; HOFFMANN Ingo; ROOSEN RUNGE Felix; Frank Schreiber; SEYDEL Tilo and ZHANG Fajun. (2015). Discerning static from transient clusters using neutron spin echo and backscattering. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-754
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