Influence of Cosolvents, Pressure, and Self-Crowding on the Sub-Nanosecond Dynamics of Globular Proteins
Recently, the influence of crowding and cosolvents was studied on the temperature and pressure dependent structure, intermolecular interaction potential and phase behavior of concentrated lysozyme solutions. Moreover, first data on the effect of self-crowding and pressure on the internal sub-nanosecond dynamics of highly concentrated lysozyme was studied by elastic incoherent neutron scattering (EINS) on the backscattering spectrometer IN13. Here we propose to extend these studies and explore the effects of chaotropic and cosmotropic osmolytes on the temperature and pressure dependence of the internal dynamics of the protein lysozyme over a range of protein concentrations, including cell-like crowding conditions. The proposed experiments will yield valuable information how various types of cosolvents are able to modulate the temperature and pressure dependent dynamic properties and hence function of biologically relevant dense protein solutions.
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WINTER Roland; AL AYOUBI Samy; CZESLIK Claus; GOLUB Maksym; PETERS Judith and SCHUMMEL Paul Hendrik. (2016). Influence of Cosolvents, Pressure, and Self-Crowding on the Sub-Nanosecond Dynamics of Globular Proteins. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-762
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