Influence of Pressure on the Sub-Nanosecond Dynamics of Cytoskeletal Proteins
Actin and tubulin are the main components of the cytoskeleton in eukaryotic cells and can be found in two main states: tubulin, the monomeric state, and the polymeric state of microtubules, on the other hand, actin, the monomeric state with its globular shape (G-actin) and the polymeric filamentous actin (F-actin). Both, F-actin and microtubules can be assembled in highly organized, supramolecular structures, the so-called bundles. We will explore the sub-ns-dynamics of tubulin, microtubules and bundles thereof, as well as of G-actin, filamentous and bundled actin. The amplitudes of the local structural fluctuations, which determine their reactivity and are expected to be very sensitive to pressure, can be seen within this time window and will be explored up to pressures of 4 kbar. From these data we will reveal the effect of pressure on the conformational dynamics and population of conformational substates of these cytoskeletal proteins. These findings will be highly relevant for understanding the effect of pressure on the dynamics of intracellular movement and help explain the marked effect pressure has on the dynamics of the cytoskeleton as observed in vivo.
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SCHUMMEL Paul Hendrik; AL AYOUBI Samy; NEITZEL Charlotte; PETERS Judith; WINTER Roland and ZELLER Dominik. (2017). Influence of Pressure on the Sub-Nanosecond Dynamics of Cytoskeletal Proteins. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-795
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