The dynamics of the cyan fluorescent protein Cerulean as function of high hydrostatic pressure
The sequencing of green fluorescent protein (GFP) in 1992 allowed for the demonstration of its use as a genetically encoded fluorescent reporter in cells (Chalfie et al., 1994). Since then, it has revolutionized the field of fluorescence imaging. The mutant Cerulean has become a popular reporter, which we studied recently as function of temperature. With the present application, we would like to evaluate the loss of protein flexibility in Cerulean with increasing pressures, as we found hints that pressure-induced structural changes lead to a better stabilization of the chromophore which is correlated with its flexibility.
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ROYANT Antoine; AUMONIER Sylvain; CARPENTIER Philippe; CLAVEL Damien; GOLUB Maksym; GOTTHARD Guillaume; PETERS Judith and SEYDEL Tilo. (2017). The dynamics of the cyan fluorescent protein Cerulean as function of high hydrostatic pressure. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-801
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