Lipid dynamics in yeast cell membranes
Cell membranes are complex lipid-protein assemblies that regulate a wide variety of cell functions from metabolism to cell-cell interactions, including the immune response. A wealth of knowledge has been produced about the biophysics and chemistry of simple lipid systems, but there have been few structural studies and even fewer dynamical studies of the complex lipid mixtures found in typical cell membranes. We recently developed methods for the production and purification of deuterated Pichia pastoris yeast lipids at large scale in collaboration with the ILL D-lab and the PSCM, and have recently also grown a number of other yeast strains at the Lund Protein Production (LP3) facility in both normal and deuterated form. We would, therefore, like to attempt the first inelastic and quasi-elastic experiments to compare the dynamical behavior of yeast lipid mixtures to the dynamics observed in simple membranes. In the first instance, we would like to measure the incoherent dynamics and characterize molecular diffusion of the total lipid extracts using non-deuterated lipids.
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WACKLIN KNECHT Hanna; DELHOM Robin; FRAGNETO Giovanna; KNECHT Wolfgang; OROZCO RODRIGUEZ Juan Manuel; Rheinstadter and SEYDEL Tilo. (2018). Lipid dynamics in yeast cell membranes. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-812
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