In situ real-time study of the diffusive dynamic arrest of proteins during crystallization
Protein crystallization plays an important role in structural biology and medicine. Different crystallization pathways have been identified, but a general understanding of the fundamental processes is still missing. Different pathways can be addressed by choosing different salts and changing its concentration. Investigating the dynamics of the proteins might offer new insights playing a key-role in the understanding of the crystallization. Based on previous successful results, we propose to investigate the dynamics during the crystallization process using the unique possibilities of inelastic fixed window scans on IN16b to significantly increase the existing dataset to obtain a comprehensive and reliable picture. Moreover, we propose a spin-echo experiment on the same samples to monitor the dynamic changes on and besides the Bragg and monomer-monomer peaks in-situ. Complementary SANS time will be used to characterize the sample structure in the final state. Following the recommendation by the previous subcommittee (college 8), the proposal is being resubmitted to college 9.
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BECK Christian; BUHL Lena; FEUSTEL Michal; GIRELLI Anita; GRABITZ Lara; GRIMALDO Marco; MAIER Ralph; MATSARSKAIA Olga; MUSIL Ivan; PREVOST Sylvain; ROOSEN RUNGE Felix; Frank Schreiber; SEYDEL Tilo and ZHANG Fajun. (2019). In situ real-time study of the diffusive dynamic arrest of proteins during crystallization. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-853
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