Differences between monoclonal antibodies: Viscosity, diffusion, and transient cluster formation
Antibodies are essential in the immune response of mammals and antibody injections are used in various therapies. In pharmaceutical applications, highly concentrated monoclonal antibody (mAb) solutions are required to obtain a significant therapeutic effect. The conflicting requirements of minimizing the injection volumes and of limiting the viscosities for subcutaneous injection make pharmaceutical research on highly concentrated mAb solutions essential. Importantly, viscosity in mAb solutions can depend sensitively on the type of mAb. Based on neutron spin-echo (NSE) spectroscopy, small angle scattering, and rheology, it has been hypothesized that loosely connected transient clusters are associated with the macroscopic viscosity. So far, the data comparing different mAbs are limited. Here, we aim to test this hypothesis of loosely connected transient clusters for series of different mAbs provided by Lonza. Their viscosities in solution significantly differ, despite the small structural differences restricted to the mAb Fab (antigen binding fragment) regions. In this way, we aim to obtain a comprehensive quantitative picture of the link between transient clusters and viscosity.
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MOSCA Ilaria; BECK Christian; GRAPENTIN Christoph; GRUNDEL Anna; HOFFMANN Ingo; MATSARSKAIA Olga; MATSARSKAIA Olga; NASRO Roody; POUNOT Kevin; Frank Schreiber and SEYDEL Tilo. (2023). Differences between monoclonal antibodies: Viscosity, diffusion, and transient cluster formation. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-932
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