Effects of extracellular vesicles on model plasma membranes dynamics
Extracellular vesicles (EVs), nanosized vesicles produced by all cells, are widely studied for their key role in mediating intercellular communication and for being responsible for the spreading of diseases as cancer. Still, their internalization mechanisms as their effects on target cells membrane are not widely explored. We are studying EVs extracted from neuroblastoma N2a cells comparing their properties to EVs isolated from the same cells after treatment with the OligoGM1 saccharide, found to have neuroprotective functions to neuronal cells. The treatment with OligoGM1 is found to affect also EVs-mediated cell-cell communication and EVs effects on target plasma membranes properties: complementary DLS, AFM, NR, SANS, FT-IR measurements show differences in the two EVs structural properties and on their mecahnisms of interaction with model membranes. DSC shows that they differently affect target membrane thermotropic behaviour and phase transition cooperativity. To shed light on EVs effects on target membrane dynamics, linked to EVs physiological function, we propose neutron spin-echo experiments on DMPC liposomes before and after interaction with the two kinds of EVs.
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The recommended format for citing this dataset in a research publication is in the following format:
RONDELLI Valeria Maria; Sally Helmy; FARAGO Bela; Alexandros Koutsioubas and PICCININI Alice. (2023). Effects of extracellular vesicles on model plasma membranes dynamics. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-969
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