Dirty SANS: A feasibility study of membrane protein structure determination using cell-free protein synthesis: a new approach
We propose to carry out proof-of-principle measurements on instrument D22 in which we test the feasibility of analysing protein structures using SANS data recorded directly from the cell-free synthesis media in which they are produced. The advantage of being able to do this is the fact that it will avoid the need to purify - a major stumbling block afflicting this field. If successful, the novel deuteration approach involved will have wide application for membrane protein structure determination - an important area where structural information is in short supply.
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JUGNARAIN Vinesh; CORTES Sandra; FORSYTH Victor Trevor; HAERTLEIN Michael; MARTEL Anne; MITCHELL Edward; PAPATHANASOPOULOS Maria and TILLIER Bruno. (2016). Dirty SANS: A feasibility study of membrane protein structure determination using cell-free protein synthesis: a new approach. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-05-426
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