Bilayer oxidation using labelled POPC to confirm chemical mechanism and structure of decay by ozone oxidation.
This proposal follows a very successful proof-of-concept study and we request time to elucidate the mechanism for the chemically more complex POPC. Oxidation of membrane lipids in biology is important because it may be a mechanism for ageing, cell apoptosis and cancer. We propose to use our skills obtained in oxidation of fatty acids and lipids at the air-water interface to investigate the oxidation of a bilayer at solid-water interface. Our oxidant (ROS) will be aqueous ozone and our bilayer will be two different isotopologues of POPC: half-deuterated oleyl chain, or fully deuterated palmitoyl chain supported on a quartz window in a flow cell. Half-deuterated Oleyl chain POPC has recently been synthesised. Neutron reflection will allow us (1) to record the kinetic decay of head only, tail only and complete lipid by selective deuteration (2) investigate the change in morphology (film thickness and integrity) of the oxidized lipid bilayer kinetically. The bilayer, oxidant, analysis and technique are either well- studied systems or systems we have used previously. We will provide kinetics, chemical mechanism and morphological data on the oxidation of a biological relevant bilayer.
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KING Martin; JONES Stephanie; RENNIE Adrian R.; THOMPSON Katherine; WARD Andy and WATKINS Erik. (2012). Bilayer oxidation using labelled POPC to confirm chemical mechanism and structure of decay by ozone oxidation.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-10-1241
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