Elucidation of the nanostructure of DNA/RNA complexes with cationic polymer brushes
Although the field of gene therapy has matured quickly and is becoming now better established clinically, a number of challenges remain. These include improvement of the stabilisation of complexes and protection of the genetic material to be delivered, as well as the enhancement of their therapeutic efficacy, over prolonged periods of time. We have previously showed that polycationic brushes decorating the surface of nanoparticles display particularly promising properties for the stable capture of RNA oligonucleotides and display high transfection efficiencies. In this proposal, we aim to elucidate the architecture of complexes formed of RNA and DNA double stranded oligonucleotides, mRNA molecules and larger double stranded DNA molecules with dense poly(dimethylaminoethyl) (PDMAEMA) brushes. This understanding will help identify how to best engineer brush architecture for the delivery of different types of RNA/DNA molecules, but also will shed lights onto how such architecture may be expected to evolve in new generations of polymer brush-based vectors, such as block copolymers.
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The recommended format for citing this dataset in a research publication is in the following format:
ZARBAKHSH Ali; CAMPANA Mario; Julien E. Gautrot and GUTFREUND Philipp. (2023). Elucidation of the nanostructure of DNA/RNA complexes with cationic polymer brushes. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-11-2092
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