Morphology and Phase Diagram of Thermoresponsive Polymer-Protein Conjugates
Using an enzyme mediated conjugation method we have prepared well-defined protein-polymer conjugates using a model protein, green fluorescent protein (GFP) with thermo-responsive polymers based on HPMA (N-2- hydroxypropyl methacrylamide)-Acetyl-HPMA (O-acetyl-N-2-hydroxypropyl methacrylamide) and a commercially available PNIPAM-NH2. In this proposal we wish to study the self-assembly of these temperature responsive polymer-protein conjugates. Such investigations are required to understand the structural properties of these materials as the molecular weight changes and how they respond to the polymers transition to a collapsed state at higher temperatures. The phase diagram of eGFP-copolymer conjugates will be mapped out with respect to copolymer composition and concentration effects as well as temperature and water content.
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Karen J Edler; EL FAGUI Amani and SCHWEINS Ralf. (2014). Morphology and Phase Diagram of Thermoresponsive Polymer-Protein Conjugates. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-12-352
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