Domain Structure and Dynamics of Target Nanoparticles for cancer therapy - Polymer and Liposomes with surface bound protein (TR-SANS)
Targeted Nanoparticles as drug carriers for cancer therapy are equipped for cell recognition by an artificial protein-ligand shell. These will be Lanthanide loaded polymer (PLGA, patent: Gutenberg-university), and liposomes (fast development system). Here PLGA and liposomes with entrapped radio-therapy (RT) enhancers (B, Gd, Er) are surface linked to serum albumin BSA through a cleavable peptide-SH-linker. BSA is recognized by cells (clinical studies). The S-bridge binding principle is general applicable. The proteo-NP’s shall be investigated in the structure and dynamics by SANS in combination with DLS in a size range of 1nm - 50ìm & time resolved TR-SANS : double-beam technique. The following structure aspects shall to be addressed: 1) How stable are nanoparticles if: a) a high concentration of target is incorporated?; b) when and how the ligand-protein is inserted (reconstitution in membranes, PLGA) ? c) upon beam-absorption in the Nanoparticles: TR-SANS + neutron absorption (B, Gd)? 2) How is the NP-ligand-protein structurally organized, especially in ligand exposition? - D-contrast variation (solvent and lipid) in SANS 3) Stabilization of the NP’s by detergent, sugar
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NAWROTH Thomas; DECKER Heinz; EFFERTH Thomas; FREY Holger; GRILLO Isabelle; HELLMANN Nadja; KREBS Lidija; LANGGUTH Peter; SCHMIDBERGER Heinz and SCHWEINS Ralf. (2014). Domain Structure and Dynamics of Target Nanoparticles for cancer therapy - Polymer and Liposomes with surface bound protein (TR-SANS). Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-12-363
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