Lipid-polymer nanodiscs stabilized with amine-based polymers
Polymer stabilized lipid nanodiscs offer enormous potential as tools for enabling membrane protein structural studies and biophysics. Previously we have studied nanodisc formation using a poly(styrene-alt-maleic acid) (PSMA) copolymer, which forms discs above pH 8. However this anionic polymer is sensitive to Ca2+ ions (common in biological solutions) and some membrane proteins prefer lower pH environments. Thus here we propose to study lipid nanodiscs stabilized using a new amine-based polymer, poly(styrene-alt-maleimide) (PSMI). Preliminary experiments demonstrate that this polymer also forms discs, which are stable at lower pH values. Recent synthesis in our group has produced PSMA using RAFT polymerisation with well-defined molecular weights. PSMI can be generated directly from this material giving PSMI with identical polymer lengths. The length of the maleimide chain on the PSMI will also be varied from propyl, to ethyl to methyl. Thus here we wish to characterise and compare PSMA-stabilized discs to our new PSMI-stabilized discs to determine whether the structures are equivalent and how the variation in polymer structure affects the discs.
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Karen J Edler; ARNOLD Tom; SCHWEINS Ralf; TERRY Ann and TOGNOLONI Cecilia. (2015). Lipid-polymer nanodiscs stabilized with amine-based polymers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-12-398
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