Direct observation of the kinetics of lipid exchange in polymer stabilised nanodiscs with and without proteins
The use of nanodiscs to contain and solubilise phospholipid bilayers and membrane proteins is becoming more widely used. This proposal aims to directly observe the kinetics of lipid exchange between polymer stabilised nanodiscs and a monolayer of lipids at the air water interface. Previous neutron reflectometry measurements have demonstrated that this exchange occurs rapidly; faster than the time required to measure a full reflectivity profile. Although SANS data has also demonstrated that exchange occurs, for technical reasons we have been unable to successfully measure exchange kinetics using that technique. Here we propose to use the unique high flux, low-Q mode of FIGARO to directly measure the rate of change in scattering as deuterated lipids move between nanodiscs and the interfacial monolayer. We will investigate this as a function of temperature, lipid and the polymer used to encapsulate the lipids. We will also directly observe whether exchange is also seen for nanodiscs made from natural E.coli lipids and containing a membrane protein. This may potentially allow manipulation of protein function via control of their surrounding lipid environment
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Karen J Edler; ARNOLD Tom; Richard A. Campbell; Stephen C. L. Hall and TOGNOLONI Cecilia. (2016). Direct observation of the kinetics of lipid exchange in polymer stabilised nanodiscs with and without proteins. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-12-457
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