Polymer interactions with phospholipid monolayers: Understanding formation of polymer-stabilized nanodiscs
Polymer stabilized phospholipid nanodiscs are gaining rapid interest as a detergent free method of extracting membrane proteins from cell membranes and maintaining them in an active state in solution. Although the structure of the discs is established, with the polymer belt wrapping the exposed hydrophobic tails of a ~10nm disc of lipid bilayer, it is not known how this structure forms from the initially intact lipid membrane. Formation speed of the discs depends on polymer molecular weight and composition but can be as fast as 20s. In this experiment we aim to use the unique high flux, low Q mode of FIGARO to follow the kinetics of adsorption and/or penetration of deuterated polymer to lipid monolayers at the air water interface, as well as to characterise the final structure of the lipid/polymer layer. We will vary the surface pressure and composition of the lipid membrane, and the polymer MW and hydrophobic/hydrophilic balance. These experiments will access timescales inaccessible on other neutron reflectometers, to improve our understanding of how polymer-stabilized nanodiscs form, which will improve their future application and uptake in membrane protein biology.
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Karen J Edler; ARNOLD Tom; Richard A. Campbell; Stephen C. L. Hall; TERRY Ann and TOGNOLONI Cecilia. (2016). Polymer interactions with phospholipid monolayers: Understanding formation of polymer-stabilized nanodiscs. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-12-458
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