Internal and hierarchical morphology of poly(2-phenyl-2-oxazoline) (HPPhOx)/DNA aqueous self-assemblies
The purpose of this proposal is to investigate using small angle neutron scattering (SANS) and contrast-variation SANS at ambient conditions the nanoscale aqueous morphology of protonated assemblies between hydrolyzed poly(2-phenyl-2-oxazoline) (abbreviated as HPPhOx) polyelectrolytes and DNA macromolecules, as a function of ionic strength, HPPhOx to DNA concentration ratio and HPPhOx molecular weight. Self-assemblies from HPPhOx have proven to be successful encapsulation scaffolds of biomacromolecules. The D33 instrument at ILL posesses large enough spatial resolution (q-range: 5 ×10-3 to 0.25 A-1)to elucidate structural features in the HPPhOx/ DNA assemblies at different length scales: such as mesh size, internal domains, fractal hierarchical structures and aggregation. D2O will be utilized as solvent to achieve large contrast with respect to the protonated macromolecules. Contrast variation by changing the H2O/D2O volume ratios will be used in order to decouple the net contribution of different constituents (separate contribution of DNA from HPPhOx) in the structuration (e.g. mesh size, fractal aggregate dimensions) of these complex polyelectrolyte assemblies.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
Vagias, Apostolos; CUBITT Robert; HEINEMANN Andre; PAPAGIANNOPOULOS Aris and PREVOST Sylvain. (2020). Internal and hierarchical morphology of poly(2-phenyl-2-oxazoline) (HPPhOx)/DNA aqueous self-assemblies. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-12-622
This data is not yet public
This data is not yet public
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.