Lipid Interaction with Styrene Maleic Acid Copolymers on Gold Nanoparticles
Polymer stabilized lipid nanodiscs can be used to extract and stabilize membrane proteins in solution for structural studies. Previously, we studied nanodisc formation using poly(styrene-maleic acid) (SMA) copolymers synthesised by RAFT techniques, which give defined weights and architectures, and also have thiol end groups that may be used for surface binding. We have now shown that SMA tethered to gold nanoparticles will solubilise lipid vesicles. By tethering SMA to relevant nanoparticle surfaces, such as conductive gold nanoparticles the applications of SMA-nanodiscs could be broadened towards sensors for protein binding or developed for use as a membrane protein separation system. However, it seems unlikely that a polymer tethered to a nanoparticle will form disc-like structures in the same way as this system does in solution. Here, we aim to use SANS and contrast variation to determine the surface structures that develop when tethered SMA polymers bind lipids in this system, and how these compare with known SMALP nanodisc morphologies. We will also vary the position of the styrene block relative to the surface to identify how this alters bound lipid structures.
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Karen J Edler; NEVILLE George and PREVOST Sylvain. (2021). Lipid Interaction with Styrene Maleic Acid Copolymers on Gold Nanoparticles. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-12-625
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