Enzyme Diffusion through a Crosslinked Polymersome Membrane
Polymersomes are fabricated using self-assembly of amphiphilic block copolymers to multicompartment-capsules, and hold great promise as synthetic cells to mimic cell functions and in the diagnostics and therapeutics of different diseases. However, complete knowledge of the molecular processes for the encapsulation and release of nanometer-sized biomolecules from tunable multiresponsive polymersomes within the physiological pH range of 4-8 is still required. Current studies on the encapsulation processes using dynamic and static light scattering or TEM deliver inly information on global size dimensions. These techniques are limited when it comes to an in depth analysis of membrane properties at the sub-nanometer scale. Thus, an essential breakthrough in the understanding the diffusion properties through the membrane depending on pH, size and charge of the cargo, is still lacking due to the very small sizes of the membrane and its amphiphilic structure. In this project, we aim at systematical SANS experiments on model polymersome-protein hybrids to understand the location of cargo, permeability of the polymersome membrane and conformation of the amphiphilic polymers in the membrane.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
Albena Lederer; KAPIL Kriti; Upenyu L. Muza; PALINSKE Max and SCHWEINS Ralf. (2021). Enzyme Diffusion through a Crosslinked Polymersome Membrane. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-12-644
This data is not yet public
This data is not yet public
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.