Investigation of RNA adhesion to functionalized nanoparticles for biomedical applications
Functionalized magnetic nanoparticles (NPs) designed to detect viral RNA (e.g. SARS-CoV-2) have been prepared. Magnetic core (Fe3O4) of the composite particle is capped by amorphous silica (SiO2) shell and the surface is modified by specific organic molecules (APTES) that bind to RNA. The system exhibit high application potential for COVID19 test efficiency enhancement or biosensor design. In the proposed experiment, RNA interaction with 3 different samples will be investigated. Flat surface (1) and monolayer of Fe3O4@SiO2 NPs (2.) capped by APTES layer after their deposition on the flat substrate. Sample 3 will contain Fe3O4@SiO2@APTES monolayer, i.e. the NPs have been modified by APTES before their deposition onto substrate. The variation in scattering contrasts and RNA concentration in the chamber will allow for assessing RNA binding, its thickness and density as well as the structure of the three examined systems and their effect on the interaction. The results will help in tailoring of our NP systems by modification of their properties (size, shape, surface) in order to obtain higher RNA affinity and thus enhanced efficiency of potential viral diagnostics tests or biosensors.
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The recommended format for citing this dataset in a research publication is in the following format:
Hrubovcak; BARUTIAK Michael; BENOVA Eva; KUCERKA Norbert; NAGY Lubos; Nicoḷ Paracini; PEDDIS Davide; SZUCSOVA Jaroslava and Zelenakova. (2023). Investigation of RNA adhesion to functionalized nanoparticles for biomedical applications. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-12-694
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