Understanding the triolein/aqueous interface and lipase activity
The triglyceride aqueous interface has an important role in many processes, none more so than lipase activity. Lipases, such as TLL, are responsible for the degradation of triglycerides to smaller components, such as fatty acids. TLL attracts considerable interest in the food and pharmaceutical industries due to the catalytic behavior it possesses. In spite of the importance of this aqueous triglyceride system for many processes, relatively little is known about the interface compared to that between alkanes and water. Thus, understanding the oil-water interface and how this influences lipolytic activity is of significant importance. Recently, we have studied the aqueous/triolein interface using coarse-grained simulations, spectroscopic ellipsometry and quartz crystal microbalance with dissipation. Although these measurements provided detailed information related to changes in the film thickness and integrity, no information is provided for the change in the internal structure and composition with solvent uptake nor where the lipase activity takes place. Therefore, we propose using neutron reflectometry, which allows both properties to be examined.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
Ben Humphreys; ARNOLD Tom; Armando Maestro and NYLANDER Tommy. (2021). Understanding the triolein/aqueous interface and lipase activity. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1005
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.