Structural organization of HoLA-containing bilayers: the effect of the hopanoid moiety
Hopanoids are membrane lipids with similar structure and function to the steroids commonly found in the eukaryotic cell membrane, i.e. cholesterol. The peculiar characteristic of Bradyrhizobium Hopanoid Lipid A (HoLA) molecule is that the hopanoid is covalently bound to the lipid A instead of being dispersed in the membrane as for most of the Gram-Negative bacteria. Vesicles were successfully prepared with POPC, POPG, HoLA or its mutant (BTAi1Dshc) without hopanoid moiety as mimics of the bacterial outer membrane. The structure and dynamic of the vesicles was preliminarily investigated, which confirmed the presence of HoLA in the vesicles. Here we propose to complete this study by performing Neutron Reflectometry (NR) measurements on asymmetric supported POPC/POPG bilayer containing HoLA and/or its mutant BTAi1Dshc alternatively in the outer bilayer leaflet. Asymmetric lipid bilayers could be prepared by Langmuir-Blodgett method, in collaboration with PSCM laboratories. NR can provide unique information on the structure of HoLA lipid bilayer.
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VITIELLO Giuseppe; CAVASSO Domenico; FRAGNETO Giovanna; GALLUCCI Noemi; NYLANDER Tommy and PADUANO Luigi. (2021). Structural organization of HoLA-containing bilayers: the effect of the hopanoid moiety. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1014
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