Influence of NSAIDs on the Phospholipid Mammalian Neuronal Membrane
Phospholipid will be extracted from porcine brain tissue as described Folch et. al. [3]. Phospholipid mixed with phosphate buffer (PBS), pH 7.4, at 0.3 wt/v% and sonicated for ~20-30min and extruded through 100nm polycarbonate membrane, 51 times, to obtain ULV. The appropriate amount of NSAIDs namely aspirin, ibuprofen and diclofenac will be mixed with phospholipid to obtain 10, 20, 30- mol% of NSAIDS. We propose NSE and high resolution TOF techniques to investigate influence of NSAIDS on the dynamics of phospholipid membranes derived from mammalian brain tissues. On one hand NSE can provide the long range membrane fluctuations under the influence of NSAIDs, A-I-D, and IN5 can reveal change in fast phospholipid motions on the molecular length scale. We also propose SANS instrument to investigate shape and structural changes in ULV membrane
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The recommended format for citing this dataset in a research publication is in the following format:
DUBEY Purushottam Shashikumar; CZAKKEL Orsolya; Henrich Frielinghaus; HOFFMANN Ingo; HOLDERER Olaf; JAKSCH Sebastian; MATSARSKAIA Olga; OLLIVIER Jacques; PREVOST Sylvain and SAHA Debasish. (2023). Influence of NSAIDs on the Phospholipid Mammalian Neuronal Membrane. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1040
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This data is not yet public
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