Nanostructural characterisation of sVE interaction with lipid bilayer membranes: basis for biosensor development
We propose to draw a mechanistic understanding of the molecular mechanisms responsible for protein/lipid interactions by performing nanostructural characterisation with neutron reflectometry. This proposal is an integral part of an INNOVAXN PhD grant, which was awarded in 2021 to Mme Beatrice Barletti for the development of a novel biosensor based on nanostructured biomimetic lipid membranes. The results from this proposal will provide essential structural characterisation for the interpretation of our initial functional investigations that demonstrated the interaction of a blood protein biomarker, soluble VE-Cadherin (sVE), with a biologically relevant lipid bilayer membrane. sVE is the glycosylated extracellular domain of the Vascular-Endothelial cadherin and is cleaved from endothelial cells following inflammation or infection, leading to a breakdown of the endothelial junctions and a loss of the endothelium¿s barrier function. Hence, the presence of sVE in the blood provides a sensitive biomarker of vascular endothelium pathophysiology and can be used for early detection of diseases associated with vascular permeability.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
BARLETTI Beatrice; ALCARAZ Jean Pierre; FRAGNETO Giovanna; MACCARINI Marco; MARTIN Donald and Nicolò Paracini. (2023). Nanostructural characterisation of sVE interaction with lipid bilayer membranes: basis for biosensor development. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1048
This data is not yet public
This data is not yet public
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.