Characterisation of the effects of osmotic stresses on the structure of hepatitis B virus (HBV) capsids using SANS with contrast variation
Packaging and release of genetic material is one of the most crucial steps in hepatitis B virus (HBV) replication cycle. In particular it is pivotal to understand how the capsid enclosing genetic material assembles and dissembles during this process. To access these information conventional methods (e.g. mass spectrometry or electron microscopy) fails since it is not possible to introduce the required osmotic stresses to initiate disassembly. Here we propose a method based on the small-angle neutron scattering and contrast variation we recently introduced to study the elastic moduli of soft colloids with dimensions of few hundreds of nanometers [J. E. Houston et al. Science Advances 8: eabn6129 (2022)]. Using partially-deuterated PEG contrast matched in pure D2O, we plan to directly measure the form factors of viral capsid under different exerted osmotic stresses. Furthermore, we want to explore the effects of different osmotic stress on the fully assembled viral capsid and its intermediate states. Our approach will open up for studies of self-assembly pathway without necessity of performing time-resolved measurements.
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The recommended format for citing this dataset in a research publication is in the following format:
Andrea Scotti; ANDRE Ingemar; Judith E. Houston; PORCAR Lionel and POTRZEBOWSKI Wojciech. (2023). Characterisation of the effects of osmotic stresses on the structure of hepatitis B virus (HBV) capsids using SANS with contrast variation. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1059
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This data is not yet public
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