Location of Enzymes in a Polymersome
Polymersomes are fabricated using self-assembly of amphiphilic block copolymers to multicompartment-capsules, and hold great promise as synthetic cells, to mimic cell functions and in the diagnostics and therapeutics of different diseases. However, complete knowledge of the molecular processes for the encapsulation and release of nanometer-sized biomolecules from tunable multiresponsive polymersomes within the physiological pH range of 4-8 is still required. Preliminary SANS measurements at ILL were performed with deuterated cargo protein in D2O. The results confirm TEM results showing an increased thickness of the membrane due to interaction with the protein. With these measurements, we were only able to determine the changes in the membrane thickness after encapsulation, but not to identify the membrane conformation changes depending on pH. Yet, an essential breakthrough in understanding the exact location of the cargo is expected by using selective deuteration and different contrast in the hydrophobic core and the hydrophilic shell of the membrane.
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The recommended format for citing this dataset in a research publication is in the following format:
Albena Lederer; Upenyu L. Muza; SCHWEINS Ralf; STANVLIET Zahn and ZHANG Kehu. (2023). Location of Enzymes in a Polymersome. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1061
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This data is not yet public
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