Nanostructural investigation on how different lipids and glycosylation can affect protein-lipid interactions.
We propose to draw a mechanistic understanding of the molecular mechanisms responsible for protein/lipid interactions by performing nanostructural characterisation with neutron reflectometry. This proposal is an integral part of an INNOVAXN PhD grant, which was awarded in 2021 to Mme Beatrice Barletti for the development of a smart biomedical biosensor based on nanostructured biomimetic lipid membranes. The results from this experiment will provide essential structural information for the interpretation of our initial functional investigations that demonstrated the interaction of glycosylated and non-glycosylated protein biomarkers with biologically relevant lipid bilayer membranes. The investigated protein will be alpha-fetoprotein which is a sensitive biomarker for liver cancer found in the blood. Particularly we will identify how different lipids and glycosylation can affect this interaction. To better discriminate the effect of glycosylation, the resulting data will be compared with data from two different previously studied proteins, sVE and BSA. the comparison between their behaviours will provide a general picture of the interaction between proteins and lipidic moieties.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
BARLETTI Beatrice; ALCARAZ Jean Pierre; FRAGNETO Giovanna; MACCARINI Marco; MARTIN Donald and Nicoḷ Paracini. (2023). Nanostructural investigation on how different lipids and glycosylation can affect protein-lipid interactions.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1084
This data is not yet public
This data is not yet public
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