Mimicking the interface between mammalian plasma membrane and extracellular matrix: chondroitin sulphate-decorated supported lipid bilayers
Several vital processes, such as signal transduction and interaction with pathogens or drugs, take place on the cell surface, and specifically at the interface between the plasma membrane (PM) and the extracellular matrix (ECM). The phospholipids such as the zwitterionic phosphatidylcholine (PC), the negatively charged phosphatidylserine (PS) and cholesterol (CHOL) are among the most abundant lipids in the PM. The ECM is an additional layer bound to the PM, which is composed of flexible carbohydrates, like chondroitin sulphate CS. CSs are over-expressed in abnormal cells such as cancer cells. We propose to perform NR measurement to determine the scattering length density profile of the CS molecules at a PC/PS/CHOL bilayer surface, from which the structure of the CS layer as function of the lipid composition can be obtained. Data will be collected at physiological pH and acidic pH to mimic the environment of cancer cells. This information is crucial to elucidate the role of the PM-ECM interplay in signalling processes both in normal and cancer cells. CS-SLBs will also represent more advanced cell surface models of great interest for interaction studies with proteins and drugs.
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The recommended format for citing this dataset in a research publication is in the following format:
LUCHINI Alessandra; FRAGNETO Giovanna; ORECCHINI Andrea; Nicoḷ Paracini; RIPANTI Francesca and ROMANO Brigida. (2023). Mimicking the interface between mammalian plasma membrane and extracellular matrix: chondroitin sulphate-decorated supported lipid bilayers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1091
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This data is not yet public
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