DOI > 10.5291/ILL-DATA.9-13-467

This proposal is publicly available since 12/02/2017

Title

The measurement of the persistence length in DNA as a function of temperature

Abstract

The persistence length of DNA is a quantity used to define the flexibility of the molecule. It is a key parameter to describing the conformation of DNA and, as a result, many biological functions of the molecule including how it packs and its interaction with proteins and membranes. The persistence length varies in the presence of denatured regions, called "bubbles", which can be created using temperature as a parameter. We propose to use SANS to follow the shape of short-length DNA with predefined sequences as a function of temperature, thus correlating the persistence length to the formation of bubbles. We plan to follow the scattering up to the temperature where the two chains of the molecule completely denature, known as the "melting" temperature, and will support our findings with quantitative modeling using the Peyrard-Bishop-Dauxois (PBD) model.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

WILDES Andrew; ANGUELOV Dimitar; S. Cuesta-Lopez; GARDEN Jean Luc; JOHNSON Mark Robert; PEYRARD Michel; PORCAR Lionel; THEODORAKOPOULOS Nikos and VALLE ORERO Jessica. (2012). The measurement of the persistence length in DNA as a function of temperature. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-467

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Metadata

Experiment Parameters

  • Environment temperature

    25 - 100 (0.1) C
  • Experiment moment

    0.001 - 0.5

Sample Parameters

  • Formula

    • DNA
  • Consistence

    solution
  • Mass

    1 - 2