DOI > 10.5291/ILL-DATA.9-13-468

This proposal is publicly available since 04/05/2018

Title

Lipid Exchange in Polymer Stabilized Lipid Nanodiscs with Varying Curvature

Abstract

Styrene-Maleic Acid Lipid Particles (SMALPs) are stable nano-aggregates of polymer and lipid which self-assemble into monodisperse discs in solution. They have been shown to solubilize membrane proteins which gives them potential to assist in membrane protein structure determination. Our recent studies of these discs suggest the disc curvature can be modified by the lipid mixtures used. Since curvature affects membrane protein structures and function we therefore wish to explore the properties of our nanodiscs by studying lipid exchange between discs of selected curvature, induced either by lipid charge or by altering tail volumes. Lipid exchange will be probed by following changes in scattered intensity with time when solutions containing h-lipid discs and d-lipid discs are mixed. Following exchange rates at different temperatures will allow the thermodynamics of lipid exchange in this polymer stabilized system to be compared with studies by others on protein stabilized nanodiscs and liposomes. This work will form the basis of future structure/function studies on proteins in lipid nanodiscs with controlled curvature.

Experimental Report

Download Data

Please note that you will need to login with your ILL credentials to download the data.

Download Data

Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

Karen J Edler; GRILLO Isabelle; HAZELL Gavin; idini; NEYLON Cameron and SCHWEINS Ralf. (2013). Lipid Exchange in Polymer Stabilized Lipid Nanodiscs with Varying Curvature. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-468

Cited by

This data has not been cited by any articles.

Metadata

Experiment Parameters

  • Environment temperature

    15-50C
  • Experiment moment

    0.005-0.3 Å-1

Sample Parameters

  • Formula

    • h-DMPC, h-DMPG, styrene-maleic acid copolymer, D2O, H2O
    • d-DMPC, d-monoolein, styrene-maleic acid copolymer, D2O, H2O
    • h-DMPC, h-monoolein, styrene-maleic acid copolymer, D2O, H2O
    • d-DMPC, d-DMPG, styrene-maleic acid copolymer, D2O, H2O
  • Consistence

    solution
  • Container

    quartz Hellma cell