Looking at the diffusion in crowded eye lens protein mixtures using a colloid-based approach
Current approaches in proteomics mainly focus at the molecular level to understand the set of protein-protein interactions. However, there is an increasing awareness that a quantitative understanding also requires comprehension of crowded protein mixtures, as the concentrated protein background strongly influences the diffusion of individual proteins as well as their effective binding constants by non-specific interactions with the surrounding proteins. We have thus started to implement a colloid-physics based approach, where we use the eye lens cell as a model system and study the interactions between the crystallin proteins in concentrated solutions and mixtures using a combination of scattering techniques and computer simulations. Here we apply NSE to measure the diffusion of beta crystallin in the presence of a concentrated solution of a second lens protein, the gamma crystallin. We will use partially deuterated gamma crystallins and contrast match them using D2O buffer. We will look at different mixing ratios at total protein concentrations similar to those in the eye lens.
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STRADNER Anna; BUCCIARELLI Saskia; FARAGO Bela; OBIOLS RABASA Marc and SCHURTENBERGER Peter. (2013). Looking at the diffusion in crowded eye lens protein mixtures using a colloid-based approach. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-474
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