Understanding the interfacial structure in lipid/dendrimer mixtures
Supported Lipid Bilayers (SLBs) are important substrates for the study of drug/polymer/nanoparticle interactions and they are commonly used in neutron reflection and other surface sensitive techniques. We have widely investigated the interaction of PAMAM dendrimers with lipid vesicles and SLBs using neutron reflection and found contradictory results depending on the protocol for vesicle fusion (pH, ionic strength, hydrogenated vs deuterated lipids in H2O or D2O) or the orientation of the interface (vertical vs horizontal up and down geometries). We have carried out an initial experiment on FIGARO which demonstrated that phase separation and gravity massively influence the interfacial properties. Despite this important finding, three issues concerning (1) the role of bound vesicles, (2) the underlying mechanism of multilayer formation and (3) the presence of a lamellar structure only on silica and not the lipid bilayers remain unanswered. We request 2 days of beam time to resolve these issues and write up the work for publication. This clarification is of outmost importance if reliable data on complex mixed systems can be reliably extracted from neutron reflection experiments.
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Richard A. Campbell; CARDENAS and JAGALSKI Vivien. (2013). Understanding the interfacial structure in lipid/dendrimer mixtures. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-485
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