Distribution of bacterial architectural protein H-NS around DNA
Nucleoid Associated Proteins (NAPs) play a key role in the compaction and expression of the prokaryotic genome. The mechanism involves stiffening of the duplex and bridging of distant, like-charged segments by protein-mediated attraction. In previous SANS work, we measured the distribution of polyamines around DNA. Here, we will focus on the distribution of a generic NAP, the bacterial protein H-NS. For this purpose, we propose SANS experiments with solvent contrast matching in order to derive the DNA-H-NS and H-NS partial structure functions. The structure functions will be interpreted in terms of a radial distribution of protein density and possible protein density correlation along the duplex in register with the phosphate moieties.
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VAN DER MAAREL Johan R C; Arluison; EGELHAAF Stefan; FORSYTH Victor Trevor; GRILLO Isabelle; GUTTULA DURGARAO and ZHANG Ce. (2013). Distribution of bacterial architectural protein H-NS around DNA. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-515
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