Crowding in ternary protein solutions
The interior of living cells is occupied by macromolecules such as proteins, which occur at a high volume fraction on the order of 30% in the aqueous solution of the cellular fluid. The issue of macromolecular crowding is therefore of primordial importance for the function of living cells [R.J. Ellis, Curr. Opin. Struct. Biol. 2001, 11, 114]. To model the situation of crowding in vitro, we propose to investigate the diffusion of protein(BSA)-protein(IgG)-water(D2O) ternary mixtures, where the proteins BSA (bovine serum albumin) and IgG (immunoglobulin) are important constituents of blood. Using our previous large QENS data sets of BSA-water and IgG-water binary mixtures and consistent modeling, we expect to be able to separate the contributions of the two proteins to the QENS spectra in the ternary mixture.
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SEYDEL Tilo; DA VELA Stefano; FEUSTEL Michal; GRIMALDO Marco; ROOSEN RUNGE Felix; Frank Schreiber and ZHANG Fajun. (2014). Crowding in ternary protein solutions. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-526
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