Cytochrome P450 Reductase in Nanodiscs: determination of parameter determining its change in conformational equilibrium
The cytochrome P450 Reductase (CPR) is a membrane bound enzyme that acts as an electron donor in a NADPH-dependent manner to all microsomal CYPs. We have shown earlier that we can extract the conformational equilibrium between the open and closed conformations of CPR in nanodiscs films using neutron reflection. Moreover, this conformational equilibrium is dependent on the presence of NADPH. However, it is not known yet whether the change in conformation is due to the actual binding of NADPH to the protein, to the change in oxidation state induced by NADPH or to both oxidation state change and NADPH binding. We have explore these issues and we still require 1 more day of beam time to unravel this question.
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CARDENAS; BARKER Robert; JAGALSKI Vivien and SOTRES Javier. (2014). Cytochrome P450 Reductase in Nanodiscs: determination of parameter determining its change in conformational equilibrium. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-543
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