The adsorption of protein containing polymer-stabilised nanodiscs at the solid-liquid interface
Nanostructured lipid aggregates offer enormous potential as tools for membrane structural biology and biophysics. Poly(Styrene-Maleic Acid) stabilized lipid nanodiscs can be easily prepared, without detergent, at low cost and can solubilize membrane proteins. In this proposal we wish to study the adsorption of polymer-stabilised lipid nanodiscs containing a membrane protein at a lipid monolayer containing a lipid with a His-tag chelating ligand on a solid-liquid interface. We wish to determine whether the protein containing discs can be close packed in a single orientation at the interface to assist in future structure determination & potentially bulk 3D crystalisation. We will use ZipA, a membrane protein involved in cell division, with a single transmembrane helix (in collaboration with Prof Dafforn's group in Birmingham) for this proof-of-principle experiment.
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ARNOLD Tom; BARKER Robert; Karen J Edler; EL FAGUI Amani and HAZELL Gavin. (2014). The adsorption of protein containing polymer-stabilised nanodiscs at the solid-liquid interface. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-547
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