Out-of-equilibrium active membranes: incorporation of bacteriorhodopsin in supported lipid bilayer
Transport process through the membrane involves specific membrane proteins, which use metabolic energy as ATP hydrolysis or photochemical reaction to process conformational changes. This protein activity breaks the fluctuation-dissipation theorem leading to out-of-equilibrium fluctuations. These active fluctuations have been widely described theoretically but less is known on the experimental point of view. In the last 15 years, we have developed a new model system (fluid floating bilayer) and original off-specular reflectivity experiments allowing us to study the fluctuations of a single floating bilayer near a substrate as well as membrane-membrane interactions. It opens a wide range of perspectives to achieve a better understanding of active membranes properties which is the purpose of a PhD financed by ILL, in collaboration between ILL. We propose here to prepare supported bilayer by GUVs spreading on an interpenetrating hydrogel composed of polyelectrolyte multilayers and agarose and to check the quality of the formed supported bilayer, and especially the lateral homogeneity which is highly important to perform x-ray off-specular reflectivity.
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charitat; BASSEREAU Patricia; DAILLANT Jean; FRAGNETO Giovanna; GERELLI YURI; VAZ Ines and WULLE Simon. (2015). Out-of-equilibrium active membranes: incorporation of bacteriorhodopsin in supported lipid bilayer. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-580
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