Cholesterol location in POPS and POPC membranes
Using Time Resolved (TR) Small Angle Neutron Scattering (SANS), we have measured cholesterol transfer between unilamellar vesicles made of POPC and POPS. While cholesterol exchange is slower in POPS, we found that the transfer of cholesterol in POPS is also surprising and suddenly near -frozen- below 50C. Such discontinuous behavior suggests a quasi-first order change which is similar in character to the well documented case of cholesterol transport between lipid vesicles when crossing the lipid's melting transition temperature. Yet this anomalous cholesterol transport behavior is occurring with lipids in the fluid phase and, to the best of our knowledge, has never been reported. Structural analysis using SANS and X-ray scattering, combined with densitometry and DSC measurements failed to reveal any underlying transition responsible for such behavior. We plan to use neutron reflectometry to investigate the location of cholesterol in deuterated POPC and deuterated POPS supported bilayer as a function of temperature in order to characterize a possible -trapped or burried- state of cholesterol in the bilayer of POPS at low temperatures that could explain our SA
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PORCAR Lionel; FRAGNETO Giovanna; GERELLI YURI and PEREZ-SALAS Ursula. (2015). Cholesterol location in POPS and POPC membranes. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-583
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