Transport of Water Across Liposome Membranes Studied by Contrast Variation Stopped-Flow Experiments
The transport of water across liposome membranes is essential to life and death of cells. In our experiment we want to address that question by rapidly mixing identical liposomes prepared once with deuterated, once with normal water in the stopped-flow cell and then following the subsequent water exchange from the interior of the liposomes by SANS. This experiment uses the unique detecting properties of neutrons and will allow us to cover the kinetics of this process in the 30 ms to 100 min range. These experiments will be done for pure DOPC liposomes and mixtures with either cholesterol or DPPC to see how these alternative membrane builders affect the membrane permeability. From these experiments we will then be able to deduce a comprehensive physico-chemical picture of how the water exchange kinetics is related to the molecular composition of the phospholipid membrane and how it can be controlled by an appropriate choice of lipids.
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GRADZIELSKI Michael; Marek Bekir; GRILLO Isabelle; HOERMANN Anja and PREVOST Sylvain. (2015). Transport of Water Across Liposome Membranes Studied by Contrast Variation Stopped-Flow Experiments. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-591
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