DOI > 10.5291/ILL-DATA.9-13-602

This proposal is publicly available since 11/02/2020

Title

Distance-dependent Conformation of Saccharides in Models of Interacting Bacteria Surfaces

Abstract

Bacterial biofilms are involved in most microbial infections and also have important impacts on industrial processes. The outermost membrane surfaces of an important bacteria class are rendered with negatively charged lipid-anchored carbohydrates. These complex sugar layers govern bacterial interactions in biofilms and it was shown that the chemical structure of the carbohydrates influences structural and mechanical properties of biofilms. In order to gain molecular-scale structural insight into the interaction of bacteria membranes we propose the use of neutron reflectometry with model membranes displaying oligosaccharides.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

SCHNECK Emanuel; BERTINETTI Luca; FRAGNETO Giovanna; LATZA Victoria and RODRIGUEZ LOUREIRO Ignacio. (2015). Distance-dependent Conformation of Saccharides in Models of Interacting Bacteria Surfaces. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-602

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Metadata

Experiment Parameters

  • Environment temperature

    30 celsius
  • Experiment energy

    2A<lambda<25A
  • Experiment moment

    0.005A^-1<q<0.3A^-1
  • Experiment res moment

    3%<dq/q<10%

Sample Parameters

  • Formula

    • hydrophobized silicon blocks
    • D2O
    • Phospholipids (DSPC)
    • PEGlipid (lipopolymer)
    • synthetic lipopolysaccharides
    • bacterial lipopolysaccharides