Adsorption of undeuterated and deuterated myoglobin on a block copolymer surface with tailored nanopores size and hydrophilicity
The aim of the proposal is the study of the physical adsorption of myoglobin in the nanopores of block copolymer (BCPs) thin films of tailored size. The small amount of studies on the effect of constrained environment on the proteins adsorption in nanopores is partly due to the lack of information relative to the exact morphology and physical-chemical features of the used porous media. Exploiting the BCPs self-assembly, we have set up a procedure that allows building nanostructured thin films with well-defined architecture containing pores delimited by hydrophilic walls. Very promising preliminary results have been obtained in neutron reflectivity measurements performed during the test experiment 2428 on FIGARO. For these reason, we intend to study more in depth the protein adsorption at the solid/liquid interface by using a fully deuterated myoglobin. Also, we intend to perform time-resolving experiments at a number of myoglobin concentrations, in order to better understand how the nanoscale morphology of our substrate influences the protein adsorption/desorption mechanism.
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MALAFRONTE Anna; AURIEMMA Finizia; BARKER Robert; CIOCE Claudia and DI GIROLAMO Rocco. (2015). Adsorption of undeuterated and deuterated myoglobin on a block copolymer surface with tailored nanopores size and hydrophilicity. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-608
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