DOI > 10.5291/ILL-DATA.9-13-650

This proposal is publicly available since 09/28/2021

Title

STRUCTURE OF POLYMER-SUPPORTED LIPID BILAYERS DERIVED FROM NATIVE CELL-MEMBRANE VESICLES

Abstract

Recently a generic method for producing polymer-supported lipid bilayers (pSLBs) directly from cell-derived native membrane vesicles (NMVs) was discovered. These pSLBs contain essentially all the naturally occurring cell-membrane components of the donor cell line or organelle while still retaining transmembrane protein mobility and activity. These surfaces offer a new paradigm in SLB-based biomimetic surfaces and bioanalytical sensor design. While fluorescence microscopy studies have indicated that there is at minimum a 5 nm hydration layer between the pSLB and the underlying substrate, the use of neutron reflectivity is expected to provide better insight into the architecture of these complex hybrid pSLBs.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

Hudson P. Pace; AGNARSSON Björn; Antonius Armanious; GERELLI YURI; HOOK Fredrik H; MICCIULLA Samantha and SCHNECK Emanuel. (2016). STRUCTURE OF POLYMER-SUPPORTED LIPID BILAYERS DERIVED FROM NATIVE CELL-MEMBRANE VESICLES. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-650

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Metadata

Experiment Parameters

  • Experiment energy

    2-20A
  • Experiment moment

    0.009 - 0.25 Å-1
  • Experiment res energy

    7%
  • Experiment res moment

    7%

Sample Parameters

  • Formula

    • Native membrane extract from Spodoptera frugiperda
    • synthetic lipids