To understand the antimicrobial activity of the salivary protein Histatin 5.
The goal of this project is to understand the antimicrobial activity of the intrinsically disordered salivary protein Histatin 5, which acts in defence against oral candidiasis caused by Candida albicans. From the literature it is still unknown how the peptide transports itself through the membrane, but it is suggested to not be an ordinary pore-opening transport, instead it has been attributed to its metal binding abilities. Studies have established that various transitional metals, such as Zn, Ni, Cu, and Fe, are intrinsically present in the saliva. In this project, we aim to investigate not only how Histatin 5 interacts with the SiO2 surface but also how Histatin 5 interacts with membranes, to be able to detect structural changes of the membrane, as well as determine how Histatin 5 inserts into the membrane. For this purpose, a combined experimental and theoretical approach will be used, where quartz-crystal microbalance with dissipation, ellipsometry, and neutron reflectivity will be utilized together with computer simulations. Thereby we will obtain a molecular understanding of system that is both of medical and academic relevance.
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Download DataThe recommended format for citing this dataset in a research publication is in the following format:
Marie Skepö; CRAGNELL Carolina; GERELLI YURI; MUKHINA Tetiana and RIELOFF Ellen. (2016). To understand the antimicrobial activity of the salivary protein Histatin 5.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-656
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